Each individual Nikon triple band fluorescence set is optimized for use with DAPI and FITC (fluorescein isothiocyanate) together with either TRITC (tetramethylrhodamine isothiocyanate) or Texas Red probes. The relevant spectral locations similar to these fluorochrome combinations range from violet excitation and blue emission to green excitation and crimson emission. http://andyfwmbr.livebloggs.com/2495916/5-simple-techniques-for-fluorescent-filter-singapore